Carry out research on cotton structural genome, gene cloning and functional verification of important traits, polyploid inheritance and molecular evolution, bioinformatics and so on.
Research on new theories and methods of molecular marker mapping of important genes, assisted breeding and transgenic gene breeding.
Carry out research and development of new genetic transformation systems, functional verification of target genes, and creation of new transgenic materials and varieties.
Through interspecific introgression, physical and chemical mutation, T-DNA insertion mutation and recurrent selection of distant hybridization, new germplasm was created, identified and used.
Hybridization and genome polyploidy are the main driving forces of genome evolution and the basis of Heterosis and polyploidy, which greatly enriches the species diversity in nature. Cotton, wheat, rape and other major grain, cotton and oil crops are typical allopolyploid species. At present, the main crop breeding is facing the problems of narrow genetic background and serious homogenization, which threatens the independent development of China's agricultural seed industry.
After the distant hybridization and polyploidization of diploid ancestral species, cotton has been significantly improved in fiber quality, fiber yield and adaptability, which is a typical embodiment of polyploid advantage. Therefore, cotton is also a model material for polyploid biology research.
The research group took allotetraploid cotton as the object to study the molecular mechanism of polyploid dominance. The main research results include:
(1) The regulation of asymmetric expression of polyploid cotton genome was revealed;Cotton is the main source of natural fiber in the world and has high economic value. In addition to its economic value, cotton is also a model system for studying genomic polyploidy and plant cell differentiation. Taking allotetraploid cotton as the research object, the candidate carried out research from the aspects of polyploid genome structure and evolution, selection, domestication and improvement of important agronomic traits, revealed the genetic basis of polyploid genome evolution, formation and domestication, and also provided theoretical basis and excellent gene resources for crop breeding and improvement.
In the past five years, he published 20 SCI research papers, including 8 research papers in journals such as Nature Biotechnology (1), Nature Genetics (2) and genome biology (1) as the first or co first author. He cited 608 times in SCI-E, including 1 paper highly cited by ESI and 672 times.
1. The asymmetry of genome evolution and the law of functional differentiation of allotetraploid cotton were found
1. The genome evolution, differentiation
and domestication of allotetraploid
cotton were studied by genome sequence
and expression analysis.
(1)
Through the comparative genomic analysis
of synthetic allotetraploid cotton,
upland cotton wild species, semi wild
species and cultivated species, the
variation sites were scanned in the
whole genome by bioinformatics method,
and the genomic structure variation and
epigenetic variation in the
domestication process were
comprehensively analyzed to reveal the
genomic and epigenomic laws of
allotetraploid upland cotton
domestication.
(2)
Through the association analysis of
structural variation and traits, the
whole genome scanning of selected
clearance sites, detect the gene regions
related to traits and their functional
genes, excavate new domestication genes,
clone important domestication genes and
analyze their regulation mechanism, so
as to provide important theoretical
guidance and technical support for
cotton molecular genetic improvement and
the cultivation of new cotton.
2. Through map based cloning, the genes
related to important agronomic traits of
cotton were cloned and their functions
were revealed.
Through
genetic mapping combined with BSA SEQ,
important agronomic traits such as
cotton leaves and fibers were located,
and in-depth functional analysis was
carried out.
3. Cloning and functional study of key
genes of oil anabolism in cottonseed.
Through
genome-wide association analysis, mining
important cotton oil metabolism genes,
improving cotton oil content and
components, and strengthening the
comprehensive utilization value of
cottonseed.